For a large number of short sequences,
try an experimental service.
Multiple sequence alignment and NJ / UPGMA phylogeny
Scoring matrix for amino acid sequences:
Scoring matrix for nucleotide sequences:
↑ Switch it to '1PAM / κ=2' when aligning closely related DNA sequences.
Gap opening penalty:
(1.0 – 5.0)
(0.0 – 1.0)
- Katoh, Rozewicki, Yamada 2017
(Briefings in Bioinformatics, in press)
MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization
- Kuraku, Zmasek, Nishimura, Katoh 2013
(Nucleic Acids Research 41:W22-W28)
aLeaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity