cbrc
MAFFT version 7

Multiple alignment program for amino acid or nucleotide sequences

All jobs are reset at 4:00AM (JST) every Sunday.

Multiple sequence alignment and NJ / UPGMA phylogeny

Input:
Paste protein or DNA sequences in fasta format.  Example

or upload a plain text file:


Help

UPPERCASE / lowercase:

Direction of nucleotide sequences:

  Beta
  Beta

Output order:

Notify when finished (optional; recommended when submitting large data):
Email address:

Advanced settings

Strategy:
Updated

Progressive methods


Iterative refinement methods

Help
Help
Help
Help

Parameters:
Scoring matrix for amino acid sequences:
Scoring matrix for nucleotide sequences:
↑ Switch it to '1PAM / κ=2' when aligning closely related DNA sequences.
Gap opening penalty: (1.0 - 3.0)
Offset value: (0.0 - 1.0)

Align unrelated segments, too? in Alpha Testing (2014/Mar)
If the input data is expected to be globally conserved but locally contaminated by unrelated segments, try 'Unalignlevel>>0' and 'Leave gappy regions'.

Unalignlevel:

↑ Default


Mafft-homologs (Collects homologs from SwissProt by BLAST and performs profile-based alignments; Protein only): Help


Number of homologs: (5 - 200)
Threshold: E = (1e-5 - 1e-40)

Plot LAST hits (DNA only):


Threshold: