Method:
NJ ← Conserved sites (115 aa)
NJ ← All gap-free sites (129 aa)
Average linkage (UPGMA) ← All sites (294 aa and gaps)
Minimum linkage ← All sites (294 aa and gaps)

Substitution model (valid when NJ is selected):
JTT
WAG Alpha
Poisson

Heterogeneity among sites (valid when JTT or WAG is selected):
Ignore (α = ∞)
Estimate
Specify; α = (0.10 - 5.0)

Bootstrap (valid for NJ):
On
Number of resampling: (5 - 1000)
(The number of sequences must be <1000 for Poisson model, or <100 for other models.)